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Biofluidics Lab, 328 Duffield Hall Cornell University, Ithaca, NY 14853 |
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Modeling a Microvessel (Andrew Darling, Chantel Lafond) Endothelial cells readily form a monolayer upon collagen surface, so we have methods to adhere collagen to microfluidic channels, to rapidly concentrate collagen to a percentage-by-weight so that it may be molded into channels, and to form microfluidic channels out of gelatin, which is denatured collagen, treated with various agents to keep it solid at 37 degrees C. |
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Concentration Gradients (Andrew Darling)Modified versions of the chemotaxis channel (see BioMEMS section) have been constructed from glutaraldehyde-processed gelatin, microbial transglutaminase-processed gelatin, and 3% collagen. These devices allow for generating concentration gradients and seeding cells in devices composed entirely of biocompatible hydrogels. |
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Angiogenesis Invasion Models (Andrew Darling, Chantel Lafond)By combining PDMS channels and bulk collagen components, it is possible to create concentration gradients in collagen too soft to form microchannels independently. As endothelial cells will only invade softer compositions of collagen in vitro, these hybrid material devices are necessary for angiogenesis modeling. |
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